One Step TUNEL Apoptosis Detection Kit (FITC)
一步法 TUNEL 細(xì)胞凋亡檢測試劑盒(綠色熒光)
MCE 一步法 TUNEL 細(xì)胞凋亡檢測試劑盒采用一步染色的方法快速檢測細(xì)胞凋亡。染色后，正常細(xì)胞基本無熒光，凋亡細(xì)胞呈綠色熒光。
Description& Advantages
MCE One Step TUNEL Apoptosis Detection Kit (FITC) provides a rapid and convenient method to detect cell apoptosis. When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180-200 bp fragments. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope or flow cytometer. The maximum excitation wavelength and emission wavelength of FITC were 488 nm and 525 nm respectively.
Storage：-20°C, 1 year Protect from light
Components
| Components | HY-K1078-20T | HY-K1078-50T |
|---|---|---|
| TdT Enzyme | 100 μL | 250 μL |
| FITC-12-dUTP Labeling Mix | 900 μL | 2×1.2 mL |
| TdT Dilution Buffer | 300 μL | 1 mL |
注：產(chǎn)品僅用于科研
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