Chloramphenicol (Synonyms: 氯霉su)
Chloramphenicol 是一種口服有效的廣譜抗生su (antibiotic)。Chloramphenicol 具有抗j (antibacterial) 活性。Chloramphenicol 抑制缺氧 A549 和 H1299 細(xì)胞中氧不穩(wěn)定轉(zhuǎn)錄因子和缺氧誘導(dǎo)因子-1α (HIF-1α)。Chloramphenicol 可抑制血管內(nèi)皮生長因子 (VEGF) 和葡萄糖轉(zhuǎn)運蛋白 1 (glucose transporter 1) 的 mRNA 水平，Z終降低 VEGF 的釋放。Chloramphenicol 可用于厭氧菌感ran和肺ai研究。
以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實
生物活性
Chloramphenicol is an orally active, potent and broad-spectrum antibiotic. Chloramphenicol shows antibacterial activity. Chloramphenicol represses the oxygen-labile transcription factor and hypoxia inducible factor-1 alpha (HIF-1α) in hypoxic A549 and H1299 cells. Chloramphenicol suppresses the mRNA levels of vascular endothelial growth factor (VEGF) and glucose transporter 1, eventually decreasing VEGF release. Chloramphenicol can be used for anaerobic infections and lung cancer research[1][2][3].
體外研究(In Vitro)
Chloramphenicol (1-100 μg/mL, 18-24 h) inhibits the HIF-1α pathway in NSCLC cells in a concentration-dependent manner[1].
Chloramphenicol (100 μg/mL, 0-24 h) induces autophagy in NSCLC cells, substantially increases the levels of autophagic biomarkers (beclin-1, Atg12-Atg5 conjugates, and LC3-II)[1].
Chloramphenicol induces abnormal differentiation and inhibits apoptosis in activated T cells[2].
Chloramphenicol can inhibit both bacterial and mitochondrial protein synthesis, causing mitochondrial stress and decreased ATP biosynthesis[3].
chloramphenicol (1-100 μg/mL) can induce matrix metalloproteinase (MMP)-13 expression and increase MMP-13 protein[3].
chloramphenicol (1-100 μg/mL) can activate c-Jun N-terminal kinases (JNK) and phosphatidylinositol 3-kinase (PI-3K)/Akt signaling, leading to c-Jun protein phosphorylation[3].
Chloramphenicol acts primarily on the 50S subunit of bacterial 70S rihosomes and inhibits peptide bond formation by suppressing peptidyl transferase activity[5].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[1]
| Cell Line: | A549 and H1299 cells |
| Concentration: | 0, 1, 10, 100 μg/mL |
| Incubation Time: | 3 h and 24 h |
| Result: | In the 3-h-treated group, the viability of A549 and H1299 cells at 100 μg/mL concentration was 97.0 ± 3.9% and 98.1 ± 5.0%, respectively. The viability of A549 cells was 102.9 ± 1.3% and 99.2 ± 0.9%, whereas the viability of H1299 cells was 103.3 ± 1.9% and 93.8 ± 4.5%, under hypoxia and treatment with CoCl2, respectively. |
體內(nèi)研究(In Vivo)
Chloramphenicol (0-3500 mg/kg, Gavage, daily, for 5 days) decreases erythrocytes and erythrocyte precursors and reduces marrow erythroid cells were at day 1 post-dosing, and returns to normal by 14 days post-dosing[4].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | Female B6C3F1 mice (12-14 weeks old)[4] |
| Dosage: | 0, 2500 and 3500 mg/kg |
| Administration: | Gavage, daily, for 5 days |
| Result: | Cessation of erythropoiesis was evident at day 1 post-dosing. A recovery was seen at day 7 post-dosing at the 2500 mg/kg dose level and at between 7 and 14 days at the 3500 mg/kg dose level. Myelotoxicity was most pronounced in the erythroid series at each dose level. Depressed femoral marrow BFU-E and CFU-E at day 1 post-dosing. All the blood and marrow parameters in the present study returned to normal by 14 days post-dosing. |
分子量：323.13
性狀：Solid
Formula：C11H12Cl2N2O5
CAS 號：56-75-7
中文名稱：氯霉su
來源：Streptomyces venezuelae
運輸條件
Room temperature in continental US; may vary elsewhere.
儲存方式
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years |
*該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)用現(xiàn)配，即刻使用。
溶解性數(shù)據(jù)
DMSO : ≥ 150 mg/mL (464.21 mM)
Ethanol : 100 mg/mL (309.47 mM; Need ultrasonic)
H2O : 3.06 mg/mL (9.47 mM; Need ultrasonic)
* "≥" means soluble, but saturation unknown.
| 濃度溶劑體積質(zhì)量 | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.0947 mL | 15.4736 mL | 30.9473 mL |
| 5 mM | 0.6189 mL | 3.0947 mL | 6.1895 mL |
| 10 mM | 0.3095 mL | 1.5474 mL | 3.0947 mL |
請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液；該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)用現(xiàn)配，即刻使用。
驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用； 以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶
- 1.
請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (7.74 mM); Clear solution
- 2.
請依序添加每種溶劑： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (7.74 mM); Clear solution
- 3.
請依序添加每種溶劑： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (7.74 mM); Clear solution
- 4.
請依序添加每種溶劑： 10% EtOH 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (7.74 mM); Clear solution
- 5.
請依序添加每種溶劑： 10% EtOH 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (7.74 mM); Clear solution
- 6.
請依序添加每種溶劑： 10% EtOH 90% corn oil
Solubility: ≥ 2.5 mg/mL (7.74 mM); Clear solution
- 7.
請依序添加每種溶劑： PBS
Solubility: 2.5 mg/mL (7.74 mM); Clear solution; Need ultrasonic
參考文獻(xiàn)
[1]. Hsu HL, et al. Chloramphenicol Induces Autophagy and Inhibits the Hypoxia Inducible Factor-1 Alpha Pathway in Non-Small Cell Lung Cancer Cells. Int J Mol Sci. 2019 Jan 3;20(1):157. [Content Brief]
[2]. Yuan ZR, et al. Chloramphenicol induces abnormal differentiation and inhibits apoptosis in activated T cells. Cancer Res. 2008 Jun 15;68(12):4875-81. [Content Brief]
[3]. Li CH, et al. Chloramphenicol causes mitochondrial stress, decreases ATP biosynthesis, induces matrix metalloproteinase-13 expression, and solid-tumor cell invasion. Toxicol Sci. 2010 Jul;116(1):140-50. [Content Brief]
[4]. Turton JA, et al. Characterization of the myelotoxicity of chloramphenicol succinate in the B6C3F1 mouse. Int J Exp Pathol. 2006 Apr;87(2):101-12. [Content Brief]
[5]. Bartlett JG. Chloramphenicol. Med Clin North Am. 1982;66(1):91-102. [Content Brief]
注：產(chǎn)品僅用于科研
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