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Resipher細(xì)胞能量代謝分析系統(tǒng)是在標(biāo)準(zhǔn)孔板中直接精確地測試樣本的耗氧量。其采用了離分辨率動態(tài)光學(xué)氧氣傳感器,對細(xì)胞無干擾。儀器設(shè)計小巧,緊湊,操作簡易。帶樣本的孔板放置在測試環(huán)境中后,直接把帶探針的蓋子直接插入即可,無需再轉(zhuǎn)移樣本,消除了環(huán)境改變的影響。
Resipher細(xì)胞能量代謝分析系統(tǒng)軟件為網(wǎng)格化設(shè)計,即對每個孔板內(nèi)的樣本—對應(yīng)分析,實現(xiàn)對每個樣本的監(jiān)測,快速、可視化地讀取和分析數(shù)據(jù)??赏ㄟ^電腦或移動終端,遠(yuǎn)程查看實時實驗數(shù)據(jù),獲悉細(xì)胞狀態(tài)。
Resipher采用高分辨率光學(xué)氧氣傳感器,測定細(xì)胞培養(yǎng)中的耗氧量及氧環(huán)境的變化。
Resipher是—款手持便攜式設(shè)備,放置 96 孔板上,置千培養(yǎng)箱中,對孔板內(nèi)的樣品進(jìn)行測試。Resipher主控制盒與無菌帶光纖探針的蓋子連接,蓋子上的探針直插入培養(yǎng)基中內(nèi)。微型探針直徑 500 µm,對細(xì)胞織無損傷。
細(xì)胞耗氧量是通過培養(yǎng)基中的氧濃度的梯度變化來直觀反應(yīng)的。通過動態(tài)掃描,讀取培養(yǎng)基中的氧濃度的梯度變化,再通過信號處理和計算得出實時的細(xì)胞耗氧量值。
數(shù)據(jù)記錄儀放置在培養(yǎng)箱外,可擴(kuò)展連8個主控制盒。軟件為網(wǎng)格化設(shè)計即 對每個孔板內(nèi)的樣本一一對應(yīng)分析,實現(xiàn)對每個樣本的監(jiān)測,可視化快速分析。
除了耗氧量外,也可以接入外接信號,獲取氧氣濃度、培養(yǎng)箱溫度、相對濕度、大氣壓、設(shè)備運(yùn)行狀態(tài)和其它環(huán)境因素等參數(shù)。
產(chǎn)品特征
高靈敏度傳感器,精準(zhǔn)度高,誤差小。
小巧緊湊,不占空間,兼容大多數(shù)培養(yǎng)箱。
精巧設(shè)計,無菌光纖探針的蓋子。置于孔板和主控制盒之間。
兼容各品牌的標(biāo)準(zhǔn) 96孔板,目前提供每孔板32通道探針。
實時連續(xù)監(jiān)測分析
即插即用式USB-C連接
非侵入式測試
可擴(kuò)展通道數(shù)
參考文獻(xiàn)
BIOMATERIALS ENGINEERING
Rabussier, G.; Bunter, I.; Bouwhuis, J. et. al. (2023) Healthy and diseased placental barrier on-a-chip models suitable for standardized studies, Acta Biomaterialia.
CELLULAR BEHAVIOR IN HYPOXIA
Smith, M.; Yang, F.; Griffiths, A. et. al. (2023) Redox and metal profiles in human coronary endothelial and smooth muscle cells under hyperoxia, physiological normoxia and hypoxia: Effects of NRF2 signaling on intracellular zinc, Redox Biology, 62, 102712.
LUNAR DUST
Chang, J.H.; Xue, Z.; Bauer, J. et. al. (2023) Artificial Space Weathering to Mimic Solar Wind Enhances the Toxicity of Lunar Dust Simulants in Human Lung Cells, Authorea.
CANCER THERAPY
Mohan, A.; Griffith, K.A.; Wuchu, F. et. al. (2023) Devimistat in combination with gemcitabine and cisplatin in biliary tract cancer: Pre-clinical evaluation and phase 1b multicenter clinical trial (BilT-04), Clin Cancer Res ,CCR-23-0036.
IMMUNOTHERAPY AND CANCER METABOLISM
Bell, H.; Huber, A.; Singhal, R. et. al. (2023) Microenvironmental ammonia enhances T cell exhaustion in colorectal cancer, Cell Metabolism, 35, 134-149.
EFFECTS OF OXYGEN AVAILABILITY ON CELL METABOLISM
Tan, J.; Virtue, S. et al. (2022) Oxygen is a critical regulator of cellular metabolism and function in cell culture. bioRxiv.
BACTERIAL ANTIBIOTIC RESISTANCE
Palomino, A., Gewurz, D., DeVine, L. et al. (2023) Metabolic genes on conjugative plasmids are highly prevalent in Escherichia coli and can protect against antibiotic treatment. ISME J. 17, 151
BIOPRINTED 3D MICROALGAE CONSTRUCTS
Dani, S., Windisch, J., XM, V. G., Bernhardt, A., Gelinsky, M., Krujatz, F., & Lode, A. (2022). Selection of a suitable photosynthetically active microalgae strain for the co-ction with mammalian cells. Frontiers in Bioengineering and Biotechnology, 10, 994134.
CANCER METABOLIC PATHWAY
Achreja, A., Yu, T., Mittal, A. et al. (2022). Metabolic collateral lethal target identification reveals MTHFD2 paralogue dependency in ovarian cancer. Nature Metabolism, 4, 1119–1137.
MITOCHONDRIAL METABOLISM IN HYPOXIC CONDITIONS
Salaroglio, I. C., Belisario, D. C., Akman, M., La Vecchia, S., Godel, M., Anobile, D. P., Ortone, G., Digiovanni, S., Fontana, S., Costamagna, C., Rubinstein, M., Kopecka, J., & Riganti, C. (2022). Mitochondrial Ros Drive resistance to chemotherapy and immune-killing in hypoxic non-small cell lung cancer. Journal of Experimental & Clinical Cancer Research, 41, 243
MITOCHONDRIAL DYSFUNCTION
Shu, D. Y., Frank, S. I., Fitch, T. C., Karg, M. M., Butcher, E. R., Nnuji-John, E., Kim, L. A., and Saint-Geniez. M. (2022) Dimethyl Fumarate Blocks Tumor Necrosis Factor-Alpha-Driven Inflammation and Metabolic Rewiring in the Retinal Pigment Epithelium. Front. Mol. Neurosci. 15:896786.
MITOCHONDRIAL ELECTRON TRANSPORT
Spinelli, J. B., Rosen, P. C., Sprenger, H. G., Puszynska, A. M., Mann, J. L., Roessler, J. M., Cangelosi, A. L., Henne, A., Condon, K. J., Zhang, T., Kunchok, T., Lewis, C. A., Chandel, N. S., & Sabatini, D. M. (2021). Fumarate is a terminal electron acceptor in the mammalian electron transport chain. Science, 374(6572), 1227–1237.
CARDIOMYOCYTE METABOLOMICS
Abouleisa, R. R., McNally, L., Salama, A. B. M., Hammad, S. K., Ou, Q., Wells, C., Lorkiewicz, P. K., Bolli, R., Mohamed, T. M., & Hill, B. G. (2021). Cell cycle induction in human cardiomyocytes is dependent on biosynthetic pathway activation. Redox Biology, 46, 102094.
CARDIOMYOCYTE METABOLOMICS
McNally, L. A., Altamimi, T. R., Fulghum, K., & Hill, B. G. (2021). Considerations for using isolated cell systems to understand cardiac metabolism and biology. Journal of Molecular and Cellular Cardiology, 153, 26–41.
MITOCHONDRIAL DYSFUNCTION
Mohiuddin, M., Choi, J. J., Lee, N. H., Jeong, H., Anderson, S. E., Han, W. M., Aliya, B., Peykova, T. Z., Verma, S., García, A. J., Aguilar, C. A., & Jang, Y. C. (2020). Transplantation of Muscle Stem Cell Mitochondria Rejuvenates the Bioenergetic Function of Dystrophic Muscle. bioRxiv.