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EASYspin Plus 組織/細胞RNA快速提取試劑盒

參考價￥780.00
具體成交價以合同協(xié)議為準(zhǔn)
  • 公司名稱杭州昊鑫生物科技股份有限公司
  • 品       牌艾德萊
  • 型       號RN2802
  • 所  在  地杭州市
  • 廠商性質(zhì)代理商
  • 更新時間2024/6/19 17:43:44
  • 訪問次數(shù)387
規(guī)格
50次780.00元1000 瓶可售
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杭州昊鑫生物科技股份有限公司成立于2009年初，是一家面向生命科學(xué)領(lǐng)域，從事科研機構(gòu)、高校、院所及生產(chǎn)企業(yè)所需科研試劑、耗材，儀器銷售和服務(wù)面向全國。

產(chǎn)品和服務(wù)涵蓋生命科學(xué)研究技術(shù)的諸多方面，提供覆蓋分子生物學(xué)、細胞生物學(xué)、植物學(xué)、生物化學(xué)、蛋白組學(xué)、免疫學(xué)等領(lǐng)域的實驗產(chǎn)品以及生物技術(shù)服務(wù)等。目前一級代理品牌有：MCE（Medchemexpress）、Biochannel、AATbio、invivogen、Abnova、Atlas、Origene、Biovision、云克?。–loud-clone）、艾德萊(Aidlab)、Cayman、Jackson、Epigentek、Prospec、Sciencell、icellbioscience（賽百慷）、hkABCbio、chondrex、Mybiosource、Abbexa、Innovrsrch、HPI、Hitobiotec、Greerlabs，Ostex，4ADI，LDN等。

經(jīng)營理念：以誠信為本，保證產(chǎn)品質(zhì)量求生存，創(chuàng)造企業(yè)核心競爭力求發(fā)展 , 堅持用戶至上、服務(wù)至上的原則 。


胎牛血清、MCE抑制劑激動劑、RNA提取試劑盒、ELISA試劑盒、重組蛋白
產(chǎn)地 國產(chǎn) 規(guī)格 50次
級別 其他 證書 ISO系列證書
RN28-EASYspin Plus 組織/細胞RNA快速提取試劑盒
適用于快速提取普通動物細胞和易裂解動物組織總RNA，使用基因組DNA清除柱技術(shù)確保有效清除gDNA殘留，不需要使用DNase消化，RNA可直接用于反轉(zhuǎn)錄熒光定量PCR，Northern-blot等下游實驗。
EASYspin Plus 組織/細胞RNA快速提取試劑盒 產(chǎn)品信息

RN28-EASYspin Plus 組織/細胞RNA快速提取試劑盒

適用于快速提取普通動物細胞和易裂解動物組織總RNA，使用基因組DNA清除柱技術(shù)確保有效清除gDNA殘留，不需要使用DNase消化，RNA可直接用于反轉(zhuǎn)錄熒光定量PCR，Northern-blot等下游實驗。


貨號#
規(guī)格
價格
RN2802
50 次
780.00 元



產(chǎn)品介紹：

本公司推出EASYspin無苯fen、氯仿RNA快速提取技術(shù)基礎(chǔ)上，又成功研發(fā)基因組DNA清除柱技術(shù)確保有效清除gDNA殘留，得到的RNA不需要DNase消化，可直接用于PCR、熒光定量PCR等實驗。裂解液迅速裂解細胞和滅活細胞RNA酶，然后裂解混合物通過一個基因組DNA清除柱，基因組DNA被清除而RNA穿透濾過。濾過的RNA用乙醇調(diào)節(jié)結(jié)合條件后,RNA在高離序鹽狀態(tài)下選擇性吸附于離心柱內(nèi)硅基質(zhì)膜， 再通過一系列快速的漂洗－離心的步驟, 去蛋白液和漂洗液將細胞代謝物，蛋白等雜質(zhì)去除， 最后低鹽的RNase free H20將純凈RNA從硅基質(zhì)膜上洗脫。


產(chǎn)品特點：

1.不使用有毒的苯fen，氯仿，beta巰基乙醇等試劑，也不需要乙醇沉淀等步驟。

2.快速，簡捷，單個樣品操作一般可在30分鐘內(nèi)完成。

3.研發(fā)成功基因組DNA清除柱技術(shù)確保有效清除gDNA殘留，得到的RNA不需要DNase消化，可直接用于PCR、熒光定量PCR等實驗。

4.多次柱漂洗確保高純度，OD260/OD280典型的比值達1.9～2.0，基本無DNA殘留,可用于RT-PCR，Northern-blot和各種實驗。



RN28 EASYspin plus 組織細胞RNA發(fā)表文章

  1. Engagement of Soluble Resistance-Related Calcium Binding Protein (sorcin) with foot-and-mouth disease virus (FMDV) VP1 inhibits type I interferon response in cells. Available online 21 May 2013


2.Overexpression of small heat shock protein 21 protects the Chinese oak 4 silkworm Antheraea pernyi against thermal stress. Journal of Insect Physiology, IP 3099 No. of Pages 7, Model 5G 14 June 2013


3.S-Adenosylmethionine-induced adipogenesis is accompanied by suppression of Wnt/b-catenin and Hedgehog signaling pathways. Mol Cell Biochem (2013)382:59–73,DOI 10.1007/s11010-013-1718-3


4.A critical role of interferon-induced protein IFP35 in the type I interferon response in cells induced by foot-and-mouth disease virus (FMDV) protein 2C. Archives of Virology 2014, DOI 10.1007/s00705-014-2147-7


5.Saikosaponin-D Attenuates Heat Stress-Induced Oxidative Damage in LLC-PK1 Cells by Increasing the Expression of Anti-Oxidant Enzymes and HSP72. The American Journal of Chinese Medicine, 2014, 42(5): 1–17


6.The Cellular Prion Protein Negatively Regulates Phagocytosis and Cytokine Expression in Murine Bone Marrow-Derived Macrophages. PLoS ONE 2014, 9(7): e102785. doi:10.1371/journal.pone.0102785


7.Critical role of eukaryotic elongation factor 1 alpha 1 (EEF1A1) in avian reovirus sigma-C-induced apoptosis and inhibition of viral growth. Archives of Virology 2015, 160:1449–1461


8.Baicalin protects sertoli cells from heat stress-induced apoptosis viaactivation of the Fas/FasL pathway and Hsp72 expression. Reproductive Toxicology 2015, Accepted 12 June 2015


9.Identification and expression of an ecdysteroid-responsive amylase from red crayfish Procambarus clarkii. Fisheries Science 2015, 81(2): 345-352


10.Small Heat Shock Proteins and Eicosanoid Pathways Modulate Caspase-1 Activity in the Fat Bodies of Antheraea pernyi. first posted online September 3, 2015; doi:


11.Inflammes-dependent regulation of IL-1b secretion induced by the virulent Mycobacterium bovis Beijing strain in THP-1 macrophages.Antonie van Leeuwenhoek 2015, DOI 10.1007/s10482-015-0475-6


12.Baicalin protects sertoli cells from heat stress-induced apoptosis via activation of the Fas/FasL pathway and Hsp72 expression. Reproductive Toxicology 2015, 57: 196-203


13.Plumbagin Ameliorates CCl4-Induced Hepatic Fibrosis in Rats via the Epidermal Growth Factor Receptor Signaling Pathway. Evidence-Based Complementary and Alternative Medicine 2015,


14.Protection of Bovine Mammary Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Cell Damage by Resveratrol. Oxidative Medicine and Cellular Longevity 2016, Article ID 2572175, 15 pages


15.Metabonomics applied in exploring the antitumour mechanism of physapubenolide on hepatocellular carcinoma cells by targeting glycolysis through the Akt-p53 pathway. Scientific Reports 2016, 6:29926 | DOI: 10.1038/srep29926


16.Hesperidin prevents lipopolysaccharide-induced endotoxicity in rats.I mmunopharmacology And Immunotoxicology 2016, 38(5): 364–371


17.A study of the damage of the intestinal mucosa barrier structure and function of Ctenopharyngodon idella with Aeromonas hydrophila. Fish Physiology and Biochemistry 2017, 43(5): 1223–1235


18.Both point mutations and low expression levels of the nicotinic acetylcholine receptor β1 subunit are associated with imidacloprid resistance in an Aphis gossypii (Glover) population from a Bt cotton 19.field in China. Pesticide Biochemistry and Physiology 2017, 141: 1-8


19.Silencing of histone methyltransferase NSD3 reduces cell viability in osteosarcoma with induction of 20.apoptosis. Oncology Reports 2017, 2796-2802


20.Acute aflatoxin B1 – Induced hepatotoxicity alters gene expression and disrupts lipid and lipoprotein 21.metabolism in rats. Toxicology Reports 2017, 4: 408-414(出口產(chǎn)品發(fā)的文章）


21.Cloning and expression of three thaumatin-like protein genes from Polyporus umbellatus. Acta Pharmaceutica Sinica B 2017, 3: 373-380


22.Structural analysis and immunomodulatory effect of polysaccharide from Atractylodis macrocephalae Koidz. on bovine lymphocytes. Carbohydrate Polymers 2017, 174: 1213-1223


23.Transcriptome profiling of spleen provides insights into the antiviral mechanism in Schizothorax prenanti after poly (I: C) challenge. Fish & Shellfish Immunology 2017, 62: 13-23


24.Front. Microbiol., 19 July 2017


25.VP2 of Infectious Bursal Disease Virus Induces Apoptosis via Triggering Oral Cancer Overexpressed 1 (ORAOV1) Protein Degradation. Front. Microbiol. 2017


26.MicroRNA-20a contributes to cisplatin-resistance and migration of OVCAR3 ovarian cancer cell line. Oncology letters. 2017, 1780-1786


27.Down-regulation of aquaporin 5-mediated epithelial-mesenchymal transition and anti-metastatic effect by natural product Cairicoside E in colorectal cancer. Molecular Carcinogenesis 2017, 11 September 2017 DOI: 10.1002/mc.22712  View/save citation


28.The impact of Zearalenone on the meiotic progression and primordial follicle assembly during early oogenesis. Toxicology and Applied Pharmacology 2017, 329: 9-17


29.Benzo[a]pyrene exposure under future ocean acidification scenarios weakens the immune responses of blood clam, Tegillarca granosa. Fish & Shellfish Immunology 2017, 63: 465-470


30.Transcriptome analysis of sex-related genes in the blood clam Tegillarca granosa. PLoS ONE 12(9): e0184584


31.Oral Administration of Lactobacillus rhamnosus GG Ameliorates Salmonella Infantis-Induced Inflammation in a Pig Model via Activation of the IL-22BP/IL-22/STAT3 Pathway. Front. Cell. Infect. Microbiol. 2017




注：本產(chǎn)品僅用于科研，不可用于臨床

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