上海篤瑪生物科技有限公司代理的品牌:試劑:Sigma、TCI、Amresco、Aldrich、Fluka、MERCK、Roche
生物試劑及耗材:R&D、Abcam、santan cruz、CST、 Cayman、ClioCell、Gibco、Invitrogen、Axygen、Costar、Gilson、Eppendorf、Nichipet、Dragonmed、GE Healthcare等
篤瑪主要經(jīng)營(yíng):ELISA試劑盒,標(biāo)準(zhǔn)品,抑制劑,染液,抗體等。
篤瑪生物外包實(shí)驗(yàn)免疫組化、TUNEL(凋亡)實(shí)驗(yàn)、原位雜交實(shí)驗(yàn)、HE染色、特殊染色、免疫印跡(WB),免費(fèi)代測(cè)ELISA實(shí)驗(yàn)
兔抗人染色體11開(kāi)放閱讀框架67多克隆抗體
Rabbit anti-human chromosome 11 open reading frame 67 polyclonal Antibody
【實(shí)驗(yàn)原理】
當(dāng)將抗原注射入實(shí)驗(yàn)動(dòng)物體內(nèi)時(shí),一系列抗體生成細(xì)胞會(huì)不同程度的與抗原結(jié)合,受抗原刺激后在血液中產(chǎn)生不同類型的抗體,這種由一種抗原刺激產(chǎn)生的抗體稱為多克隆抗體。多克隆抗體中不同的抗體分子可以以不同的親和能力與抗原分子表面不同的部分—抗原決定簇相結(jié)合。
將抗原導(dǎo)入敏感動(dòng)物體內(nèi)后,可刺激網(wǎng)狀內(nèi)皮細(xì)胞系統(tǒng),尤其是淋巴結(jié)和脾臟中的淋巴細(xì)胞大量增殖。如圖所示,實(shí)驗(yàn)動(dòng)物對(duì)初次免疫和二次免疫的應(yīng)答有明顯的不同。通常初次免疫應(yīng)答往往比較弱,尤其是針對(duì)于易代謝,可溶性的抗原。*注射后大約7天,在血清中可以觀察到抗體但抗體的濃度維持在一個(gè)較低的水平,在大約10天左右抗體的滴度會(huì)達(dá)到zui大值。但同種抗原注射而產(chǎn)生的二次免疫應(yīng)答的結(jié)果明顯不同,和初次免疫應(yīng)答相比抗體的合成速度明顯增加并且保留時(shí)間也長(zhǎng)。
免疫應(yīng)答的動(dòng)力學(xué)結(jié)果取決于抗原和免疫動(dòng)物的種類,但初次和二次免疫應(yīng)答之間的關(guān)系是免疫應(yīng)答的一個(gè)重要特點(diǎn)。三次或以后的抗原注射所產(chǎn)生的應(yīng)答和二次應(yīng)答結(jié)果相似:抗體的滴度明顯增加并且血清中抗體的種類和性質(zhì)發(fā)生了改變,這種改變被稱為免疫應(yīng)答的成熟,具有重要的實(shí)際意義。通常在抗原注射4-6周后會(huì)產(chǎn)生具有高親和力的抗體。
兔抗人染色體11開(kāi)放閱讀框架67多克隆抗體
Rabbit anti-human chromosome 11 open reading frame 67 polyclonal Antibody
【實(shí)驗(yàn)材料】
⒈ 實(shí)驗(yàn)動(dòng)物
成年兔。
⒉ 實(shí)驗(yàn)器材
特制兔盒;刀片;25G針頭;1ml注射器;20 ml 血液收集管;藥鏟;離心機(jī)以及塑料離心管;加樣器及加樣管;燒杯。
供應(yīng)產(chǎn)品信息
Rabbit anti-human basigin (Ok blood group) polyclonal Antibody
兔抗人basigin(OK血型)多克隆抗體
Rabbit anti-human bone marrow stromal cell antigen 2 polyclonal Antibody
兔抗人骨髓基質(zhì)干細(xì)胞抗原2多克隆抗體
Rabbit anti-human BTB (POZ) domain containing 1 polyclonal Antibody
兔抗人BTB(POZ)含1多克隆抗體域
Rabbit anti-human BTB (POZ) domain containing 10 polyclonal Antibody
兔抗人BTB(POZ)含10多克隆抗體域
Rabbit anti-human beta cellulin,BTC polyclonal Antibody
兔抗人β動(dòng)物纖維素,BTC多克隆抗體
Rabbit anti-human biotinidase polyclonal Antibody
兔抗人生物素酶多克隆抗體
Rabbit anti-human B-cell translocation gene 1, anti-proliferative polyclonal Antibody
兔抗人B細(xì)胞易位基因1、抗proliferative多克隆抗體
Rabbit anti-human B-cell translocation gene 4 polyclonal Antibody
兔抗人B細(xì)胞易位基因4多克隆抗體
Rabbit anti-human Bruton agammaglobulinemia tyrosine kinase polyclonal Antibody
兔抗人Bruton丙種酪氨酸kinase多克隆抗體
Rabbit anti-human butyrophilin, subfamily 2, member A1 polyclonal Antibody
兔抗人butyrophilin,亞科2,A1多克隆抗體成員
Rabbit anti-human butyrophilin, subfamily 2, member A2 polyclonal Antibody
兔抗人butyrophilin,亞科2,A2多克隆抗體成員
Rabbit anti-human butyrophilin, subfamily 3, member A3 polyclonal Antibody
兔抗人butyrophilin,亞科3,A3多克隆抗體成員
Rabbit anti-human budding uninhibited by benzimidazoles 1 homolog beta (yeast) polyclonal Antibody
兔抗人buddinguninhibitedbybenzimidazoles1homologbeta(yeast)多克隆抗體
Rabbit anti-human budding uninhibited by benzimidazoles 3 homolog (yeast) polyclonal Antibody
兔抗人buddinguninhibitedbybenzimidazoles3homolog(yeast)多克隆抗體
Rabbit anti-human BUD31 homolog (S. cerevisiae) polyclonal Antibody
兔抗人BUD31同源(酵母)多克隆抗體
Rabbit anti-human blood vessel epicardial substance polyclonal Antibody
兔抗人blood血管心外膜substance多克隆抗體
Rabbit anti-human brix domain containing 1 polyclonal Antibody
兔抗人brixdomaincontaining1多克隆抗體
Rabbit anti-human brix domain containing 2 polyclonal Antibody
兔抗人brixdomaincontaining2多克隆抗體
Rabbit anti-human basic leucine zipper and W2 domains 1 polyclonal Antibody
兔抗人basic亮氨酸拉鏈蛋白和W2域1多克隆抗體
Rabbit anti-human chromosome 10 open reading frame 119 polyclonal Antibody
兔抗人染色體10開(kāi)放閱讀框架119多克隆抗體
Rabbit anti-human chromosome 11 open reading frame 49 polyclonal Antibody
兔抗人染色體11開(kāi)放閱讀框架49多克隆抗體
Rabbit anti-human chromosome 11 open reading frame 51 polyclonal Antibody
兔抗人染色體11開(kāi)放閱讀框架51多克隆抗體
Rabbit anti-human chromosome 11 open reading frame 67 polyclonal Antibody
Rabbit anti-human chromosome 11 open reading frame 70 polyclonal Antibody
兔抗人染色體11開(kāi)放閱讀框架70多克隆抗體
Rabbit anti-human chromosome 11 open reading frame 73 polyclonal Antibody
兔抗人染色體11開(kāi)放閱讀框架73多克隆抗體
Rabbit anti-human chromosome 12 open reading frame 11 polyclonal Antibody
兔抗人染色體12開(kāi)放閱讀框架11多克隆抗體
Rabbit anti-human chromosome 13 open reading frame 18 polyclonal Antibody
兔抗人染色體13開(kāi)放閱讀框架18多克隆抗體
Rabbit anti-human chromosome 14 open reading frame 133 polyclonal Antibody
兔抗人染色體14開(kāi)放閱讀框架133多克隆抗體
Rabbit anti-human chromosome 14 open reading frame 166 polyclonal Antibody
兔抗人染色體14開(kāi)放閱讀框架166多克隆抗體
Rabbit anti-human chromosome 14 open reading frame 94 polyclonal Antibody
兔抗人染色體14開(kāi)放閱讀框架94多克隆抗體
Rabbit anti-human chromosome 16 open reading frame 14 polyclonal Antibody
兔抗人染色體16開(kāi)放閱讀框架14多克隆抗體
Rabbit anti-human chromosome 16 open reading frame 70 polyclonal Antibody
兔抗人染色體16開(kāi)放閱讀框架70多克隆抗體
Rabbit anti-human chromosome 17 open reading frame 28 polyclonal Antibody
兔抗人染色體17開(kāi)放閱讀框架28多克隆抗體
Rabbit anti-human chromosome 18 open reading frame 55 polyclonal Antibody
兔抗人染色體18開(kāi)放閱讀框架55多克隆抗體
Rabbit anti-human chromosome 18 open reading frame 8 polyclonal Antibody
兔抗人染色體18開(kāi)放閱讀框架8多克隆抗體
Rabbit anti-human chromosome 19 open reading frame 10 polyclonal Antibody
兔抗人染色體19開(kāi)放閱讀框架10多克隆抗體
Rabbit anti-human chromosome 19 open reading frame 43 polyclonal Antibody
兔抗人染色體19開(kāi)放閱讀框架43多克隆抗體
Rabbit anti-human C1D nuclear receptor co-repressor polyclonal Antibody
兔抗人C1D核受體輔抑制物多克隆抗體
Rabbit anti-human C1GALT1-specific chaperone 1 polyclonal Antibody
兔抗人C1GALT1特異性伴侶1多克隆抗體