說明書下載ECTX.rar 分類名稱 : 高效藥物篩選 產(chǎn)品介紹 : 測(cè)定方法 快速熒光素/熒光素酶發(fā)光法檢測(cè)細(xì)胞活性、細(xì)胞增殖、細(xì)胞毒性,用于抗癌藥物的高通量篩選。 Description Adenosine 5-triphosphate (ATP) is the chemical energy for cellular metabolism and is often referred to as “energy currency" of the cell. ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery.our EnzyLightTM Cytotoxicity Assay Kit provides a rapid method to measure intracellular ATP, cell viability and cytotoxicity. The single working reagent lyses cells to release ATP, which, in the presence of luciferase, immediay reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration and hence number of living cells.This non-radioactive, homogeneous cell-based assay can be conveniently performed in microplates. The reagent is compatible with all culture media and liquid handling systems for high-throughput screening applications in 96-well and 384-well plates. Key features Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay). Sensitive and accurate. As low as 50 cells can be quantified.Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.Robust and amenable to HTS: Z’ factors of 0.6 to 0.7 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Applications Cell proliferation: effects of cytokines, growth factor, nutrients.Cytotoxicity and apoptosis: evaluation of toxic compounds, anti-cancer antibodies, toxins, environmental pollutants etc. Drug discovery: high-throughput screening for anticancer drugs. Storage conditions: store all reagents at -20°C. Shelf life of at least 6 months. 其他 :編號(hào):ECTX-048,ECTX-100 測(cè)試:48T,100T |
北京鴻躍創(chuàng)新科技有限公司是一家經(jīng)營(yíng)科研產(chǎn)品代理、銷售生物試劑、生物耗材、生物儀器等于一身的生物技術(shù)企業(yè),其產(chǎn)品覆蓋了生命領(lǐng)域從分子生物學(xué)研究、蛋白表達(dá)、細(xì)胞培養(yǎng)到層析純化,從抗原抗體、成套試劑盒到生物培養(yǎng)及其生物活性研究等相關(guān)試劑幾近所有過程.
在生物公司林立的今天,鴻躍公司始終堅(jiān)持以“*,信譽(yù)*”的服務(wù)宗旨.憑借其對(duì)市場(chǎng)的充分了解,對(duì)產(chǎn)品深入細(xì)致的分析,以其雄厚的資本,快速的流通方式,隨時(shí)為客戶提供*、技術(shù)含量高、操作方便快捷的實(shí)驗(yàn)急需產(chǎn)品.
鴻躍公司在為廣大科學(xué)工作者們提供產(chǎn)品的同時(shí)也注重公司員工自身的素質(zhì)培養(yǎng).不但要求員工有扎實(shí)的專業(yè)基礎(chǔ),還要有較高的文化素質(zhì)和道德修養(yǎng).強(qiáng)調(diào)員工與客戶建立良好的合作關(guān)系,公司內(nèi)部的團(tuán)隊(duì)合作精神.公司規(guī)模不求較大,但求Z佳,價(jià)格不求Z低,但求合理.
總之,鴻躍公司將以“更加執(zhí)著地追求客戶的滿意度”為宗旨,全心全意地為客戶做好每一件事.同是,對(duì)一直以來關(guān)心鴻躍公司的朋友們深表感謝!讓我們與生命科學(xué)攜手共步美好的明天!
經(jīng)營(yíng)范圍
現(xiàn)貨提供兩千多種包括Sigma、Aldrich、Fluka、 Amresco、Merck、Roche、Serva、Promega、Pharmacia、 Cambrex(BioWhittaker)、Gibco、HyClone、BMA、Qbiogene、Oxid、BioLabs、Acros、Difco、等國(guó)外*公司的產(chǎn)品,并承擔(dān)以上公司專項(xiàng)訂貨業(yè)務(wù)。
二.分子生物學(xué)成套試劑盒
現(xiàn)貨提供美國(guó)Omega、Qbiogene(Bio101)、Promega核酸分離和純化試劑盒,自產(chǎn)試劑盒。期貨提供德國(guó)Qiagen、MoBiTec、美國(guó)Ambion試劑盒。
三.免疫學(xué)試劑及檢測(cè)試劑盒
*現(xiàn)貨提供三千多種Sigma、R&D、Zymail、UsBio、Santa Curz、Maixin-Bio進(jìn)口分裝抗體及免疫組化配套試劑。Biosoure、R&D 、Diaclone等公司進(jìn)口分裝Elisa Kit、Eli-spot Kit。Biotium分子探針。各種國(guó)產(chǎn)及進(jìn)口分裝二抗,國(guó)產(chǎn)檢測(cè)試劑盒及細(xì)胞因子等。
期貨提供產(chǎn)品包括Santa Cruz、Calbiochem等公司的大包裝抗體和 Maixin-Bio、Affiniti、Neomarkers、Genx BioSciense、Zyman、UsBio等公司的抗體;KPL、Santa Cruz等公司的二抗;Maixin-Bio等公司的免疫組化配套試劑;Diaclone、 IBL、 MBL、Endogen、DSL、Biosource等公司的Elisa Kit;Diaclone公司的Eli-spot Kit;Biotium、MoiecularProbe公司探針;Peprotech,、R&D、Sigma、Calbiochem等公司的細(xì)胞因子。
四.細(xì)胞生物學(xué)產(chǎn)品
現(xiàn)貨部分提供美國(guó)Gibco、HyClone、Oxoid公司的培養(yǎng)基、血清、等培養(yǎng)試劑。并承擔(dān)以上公司專項(xiàng)訂貨業(yè)務(wù)。
五.試驗(yàn)耗材
現(xiàn)貨提供德國(guó)Sartorius,英國(guó)Whatman等過濾及超濾產(chǎn)品?,F(xiàn)貨提供美國(guó)Corning Costar、B.D、日本IWAKI、比利時(shí)Orange、丹麥NUCK、德國(guó)Greiner等細(xì)胞培養(yǎng)耗材?,F(xiàn)貨提供美國(guó)Axygen、Qsp、德國(guó)Brand、SCHOTT、芬蘭Biohit、美國(guó)Fisher等常規(guī)耗材。現(xiàn)貨提供各種國(guó)產(chǎn)耗材。
六.實(shí)驗(yàn)儀器及設(shè)備
現(xiàn)貨提供進(jìn)口原裝芬蘭百得(Biohit),雷勃,大龍移液產(chǎn)品;德國(guó)Brand、Eppendorf;法國(guó)Gilson;日本Nichiryo等國(guó)移液設(shè)備。提供各種進(jìn)口及國(guó)產(chǎn)實(shí)驗(yàn)儀器及設(shè)備。
- 面議在線詢價(jià)
產(chǎn)品名稱參考價(jià)