說(shuō)明書下載ECLC.rar
分類名稱 : 血液尿液化學(xué),代謝產(chǎn)物分析 產(chǎn)品介紹 :
測(cè)定方法 比色法(565nm)定量測(cè)定L -乳酸的濃度。
產(chǎn)品說(shuō)明 乳酸是在缺氧或厭氧條件下由乳酸脫氫酶(LDH)催化生成。因此乳酸濃度的監(jiān)測(cè)是組織內(nèi)氧需求與利用平衡的良好指標(biāo)。同時(shí)對(duì)細(xì)胞和動(dòng)物生理學(xué)研究也很重要。簡(jiǎn)單,直接和自動(dòng)化的乳酸濃度檢測(cè)方法是非??扇〉?。博世生物技術(shù)提供的L-乳酸測(cè)試盒是基于乳酸脫氫酶催化氧化乳酸,其中形成的NADH耦合到甲臜(MTT)發(fā)色體上。產(chǎn)物的顏色強(qiáng)度(565nm)與樣品中乳酸的濃度成正比。
適用范圍 直接檢測(cè):樣品如血清、血漿、細(xì)胞培養(yǎng)物中的乳酸濃度。
產(chǎn)品特點(diǎn) 敏感、準(zhǔn)確: 96-孔板檢測(cè)范圍為0.05~2 mM乳酸,對(duì)于含有酚紅的細(xì)胞培養(yǎng)物樣品,檢測(cè)范圍為0.1 mM~1 mM 乳酸。方便: 僅需添加單一工作試劑并在開始和20分鐘時(shí)讀取光密度。室溫下檢測(cè),無(wú)須37℃加熱器。高通量: 通過(guò)96-孔板高通量的自動(dòng)化檢測(cè),每天可以檢測(cè)上千份樣品。
貯藏條件:所有試劑均于-20°C保存。
Description Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. our EnzyChromTM lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.
Applications Direct Assays: lactate in serum, plasma, and cell media samples.
Key features Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM L-Lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM L-Lactate in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed.High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Storage conditions. Store all reagents at -20°C. Shelf life of at least 6 months (see expiry dates on labels).
其他 :
編號(hào):ECLC-048,ECLC-100
測(cè)試:48T,100T