5-TAMRA Picolyl Azide|5-TAMRA Picolyl N3

5-TAMRA Picolyl Azide|5-TAMRA Picolyl N3
TAMRA 熒光染料是在用的多種不同共軛物中Z受歡迎的熒光試劑之一，可標(biāo)記蛋白質(zhì)和寡核苷酸。TAMRA具有吸光的性質(zhì)，它的光譜與幾類常用的熒光化合物重疊，TMR是明亮的橙色熒光基團(tuán)。

5-TAMRA Picolyl Azide|5-TAMRA Picolyl N3

 

5-tamra吡啶甲基疊氮是一種先進(jìn)的熒光探針，采用銅螯合基序提高銅的有效濃度（我）在反應(yīng)位點(diǎn)。螯合作用的吡啶甲基疊氮基團(tuán)在反應(yīng)部位提高銅的有效濃度是如此之大，導(dǎo)致高達(dá)40倍的信號(hào)強(qiáng)度的增加。此外，代替?zhèn)鹘y(tǒng)的5-tamra疊氮化物5-tamra吡啶甲基疊氮的使用允許至少十倍減少銅催化劑的濃度不犧牲標(biāo)記效率。

雖然5的混合異構(gòu)體（6）- TAMRA是*，經(jīng)常使用的紅色熒光染料染色的蛋白質(zhì)，肽和核苷酸標(biāo)記TAMRA混合異構(gòu)體可能麻煩由于顯著的信號(hào)在HPLC純化展寬。肽和核苷酸標(biāo)記與一個(gè)單一的異構(gòu)體發(fā)生通常會(huì)得到更好的分辨率在HPLC純化，往往在接合過程中需要。

總之，一個(gè)甲基吡啶基團(tuán)為疊氮探針的引入在炔烴的檢測(cè)靈敏度大幅提高。這對(duì)于探測(cè)低豐度目標(biāo)或顯著提高信號(hào)強(qiáng)度是特別有價(jià)值的。

    

Abs/Em Maxima	545/576
Extinction coefficient	91,000
Flow cytometry laser line	532 or 555 nm
Microscopy laser line	532 or 555 nm
Spectrally similar dyes	Alexa Fluor® 546, Atto™ 543, CF™ 555 Dye, DyLight™549
Molecular weight	808.89
CAS	N/A
Solubility	DMSO, DMF, MeOH
Purity	>97% (HPLC)
Appearance	Red solid
Storage conditions	-20C
Shipping conditions	Ambient temperature

5-TAMRA Picolyl Azide|5-TAMRA Picolyl N3

• 疊氮化合物(Azides)
  Azidobutyric acid NHS ester
• Azido dyes, available as solids, and as ready-to-use 10 mM DMSO solutions
• Water-soluble Cy3 azide
  Water-soluble Cy5 azide
  Cy3 azide
  Cy3.5 azide
  Cy5 azide
  Cy5.5 azide
  Cy7 azide
  Cy7.5 azide
  5-FAM azide
  6-FAM azide
  5-JOE azide
  5-R110 azide
  6-R110 azide
  6-ROX azide
  5-TAMRA azide
  炔烴類染料(Alkynes)
  Alkyne phosphoramidite, 5'-terminal
  Alkyne amidite, hydroxyprolinol
  Pentynoic acid STP ester
  熒光激活染料(Catalysts and activators)
  Ascorbic acid
  Copper(II)-TBTA plex, 10 mM in 55% a. DMSO
  
  SYBR Green I
  Gel staining solution, 10000x
  For PCR, 100x solution
  
  改良三磷酸鹽(Modified triphosphates)
  Ami1-ddUTP
  Ami1-dUTP
  
  脂類活性染料(Dye activated esters)
  Cy3 NHS ester (water soluble)
  Cy5 NHS ester (water soluble)
  Cy3 NHS ester
  Cy3.5 NHS ester
  Cy5 NHS ester
  Cy5.5 NHS ester
  Cy7 NHS ester
  Cy7.5 NHS ester
  ROX NHS ester, pure 6- isomer
  
  羰基活性染料(Dye hydrazides)
  Cy5 hydrazide
  
  巰基反應(yīng)性染料(Dye maleimides)
  Cy5 maleimide
  Cy5.5 maleimide
  
  2D DIGE熒光染料試劑盒(2D DIGE dye kits)
  Phosporamidites
  Alkyne phosphoramidite, 5'-terminal
  Alkyne amidite, hydroxyprolinol
  DMS(O)MT aminolink amidite
  Long trebler amidite
  Pyrene dU amidite