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北京鴻躍創(chuàng)新科技有限公司
主營產(chǎn)品： omega試劑盒、耗材、移液器 |
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北京鴻躍創(chuàng)新科技有限公司
主營產(chǎn)品： omega試劑盒、耗材、移液器 |
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| 分類名稱 : 血液尿液化學(xué),代謝產(chǎn)物分析 產(chǎn)品介紹 : 測定方法 比色法（570nm）或熒光法（585nm/530nm）定量測定氨/銨離子的濃度。 產(chǎn)品說明：丙酮酸在細(xì)胞代謝中起關(guān)鍵作用。丙酮酸可以通過把葡萄糖轉(zhuǎn)化為碳水化合物，通過乙酰輔酶A轉(zhuǎn)化為脂肪或能量，也可轉(zhuǎn)化為丙氨酸和乙醇。人體內(nèi)丙酮酸的非正常水平與肝臟疾病和代謝疾病有關(guān)。簡單、直接、自動(dòng)化測量丙酮酸濃度的手段，在研究和藥物研發(fā)中受到廣泛的應(yīng)用。我公司生產(chǎn)的丙酮酸測試方法使用單一工作試劑將丙酮酸氧化和過氧化氫的檢測一次性完成。在波長為570nm檢測的顏色強(qiáng)度或 lem/ex = 585/530nm處的熒光強(qiáng)度與樣品中丙酮酸濃度成正比。 產(chǎn)品特點(diǎn) 靈敏、準(zhǔn)確：只需10 mL樣本。96孔板中檢測范圍：比色法2 - 500 mM， 熒光法0.2 - 50 mM丙酮酸。簡單、方便： 該過程包含了添加單一工作試劑，在溫室下培養(yǎng)30分鐘。改良了試劑的穩(wěn)定性：優(yōu)化的配方，大大增加了試劑和信號(hào)穩(wěn)定。 產(chǎn)品用途: 可直接檢測生物樣品中的丙酮酸。用于藥品和藥理學(xué)研究，檢測藥物對(duì)丙酮酸代謝的影響。 儲(chǔ)存條件： 冰運(yùn)，在 -20°C下儲(chǔ)藏。 Description Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery. our pyruvate assay uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at lem/ex = 585/530nm is directly proportional to pyruvate concentration in the sample. Key features Sensitive and accurate. Use as little as 10 mL samples. Linear detection range in 96-well plate: 2 to 500 mM (17 mg/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 mM for fluorimetric assays.Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays.Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Applications: Direct Assays: pyruvate in biological samples.Drug Discovery/Pharmacology: effects of drugs on pyruvate metabolism. Storage conditions. The kit is shipped on dry ice. Store all reagents at -20°C. Shelf life of three months after receipt. 其他 :編號(hào)：EPYR-048，EPYR-100 測試：48T,100T |
