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詳細介紹
德國徠卡DFC365FX產(chǎn)品介紹：
Leica DFC365 FX---High-end Scientific Fluorescence CCD CameraThe Leica DFC365 FX offers excellent fluorescence documentation and fast time-lapse recordings under low light conditions. Based on the state-of-the-art Sony EXview HAD ICX285 sensor®, this cooled camera is exceptionally well suited for a wide range of fluorescence applications from basic fluorescence imaging to demanding applications such as TIRF, FRET, and Structured Illumination.
superior Image Quality
The new Leica DFC365 FX provides anexcellent signal-to-noise ratio due to theregulated one-stage Peltier cooling of the sensor, which is highly effective even under live cell temperature conditions of 37°C. The read-out speed of the sensor can be down-regulated to 1.6 MHz for further noise reduction if eeded. Any residual noise within the unlabeled background of the specimen is easily eliminated using the camera's “Black Balance” that resets a definable gray value to zero. The settings are absolutely reproducible and can be fine-tuned by the user.
Extended versatility
The Leica DFC365 FX offers two acquisition modes: Standard and NIR (near infrared). The NIR mode is the better choice when increased quantum efficiency is needed in the spectral range starting at a wavelength of 700 nm. This widens the selection of fluorescence markers to include some that may not be suitable for imaging with standard CCD cameras. Also, this optional imaging mode protects live cells from photo-damage by offering reduced exposure times.
Maximum depth of detail
Fluorescence cameras generally offer a broad dynamic range to ensure that dark and bright signals with all details are captured within the same image.
This means that tiny differences in fluorescence signal can be detected without time-consuming HDR (High Dynamic Range) multiple image acquisition. With a dynamic range of more than 69 dB, the Leica DFC365 FX covers all application requirements. No image information is lost, and very bright ignals as well as black background can be monitored without missing details.
Posterior spiracles of a 3rd instar Drosophila melanogaster larva. Maximum projection of 45 optical planes (z distance 1.5 μm) using 20x Plan Apo objective (NA = 0.6). Autofluorescence after excitation of light with the wavelengths 488 nm and 545 nm.
Sample: Courtesy of Prof. Stephan Sigrist, Free University Berlin, Germany.
Labium of an adult Drosophila melanogaster. 3D reconstruction of 48 optical planes (z distance 1.5 μm) using 20x Plan Apo objective (NA = 0.6). Green: Titin-GFP, a muscle protein. Red: autofluorescence of Chitin.
Sample: Courtesy of Prof. Dr. Ralf Jacob, University Marburg, Germany.
Cortical neuronal cells. Maximum projection of 21 optical planes (z distance 0.5 μm) acquired with 40x Plan Fluotar L objective (NA = 0.7). Deconvolved using the
Blind Deconvolution algorithm. Red: Cy5 staining of βIII-tubulin. Green: Cy2 staining of Nestin, a marker of neuronal stem cells. Purple: Cy3 staining of DCX, a marker of immature neurons. Blue: DAPI, stains the nuclei.
Section of rat brain. Maximum projection of 26 optical planes (z distance 1.5 μm) acquired with 40x Plan Fluotar L objective (NA = 0.7), "Black Balance" applied. Deconvolved using the Blind Deconvolution algorithm. Red: Cy2 staining of GFAP (glial fibrillary acidic protein). Green: Cy5 staining of NeuN, a marker of mature neurons. Purple: Cy3 staining of DCX, a marker of immature neurons. Blue: DAPI, nuclei.
Both samples provided by FAN GmbH, Magdeburg, Germany.
德國徠卡DFC365FX基本參數(shù)：
| Digital camera Leica DFC365FX Technical Data | |
|---|---|
| Camera type | Digital monochrome, high-sensitivity cooled camera for fluorescence microscopy |
| Housing | Aluminum, Size (L × W × H) 132 mm × 74 mm × 71 mm |
| Weight | 572 g |
| CCD sensor | Sony ICX285, EXview HAD CCD technology, 2/3“ interline transfer progressive scan CCD |
| Number of pixels | 1392 x 1040 |
| Pixel size | 6.45 μm × 6.45 μm |
| Sensitive surface | Sensitive surface |
| Full well capacity | 18.000 electrons typical |
| Exposure time | 7 μseconds – 10 minutes * |
| Cooling | One-stage Peltier cooling |
| Dark noise | 6 electrons typical (1.6 MHz, 12 bit) |
| Dark current | < 0.5 electrons/pixel/second |
| Dynamic range | > 69 dB typical (1.6 MHz) |
| Operating modes | Standard mode and NIR mode |
| A/D converter | 14 bit |
| Bit depth | 8 bit/12 bit |
| Partial scan | Freely definable ROI (region of interest), combination with binning possible |
| Analog gain | 1× – 10× |
| C-mount | 0.7 x |
| Data | IEEE 1394b FireWire 9-pin single cable |
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