Cytochalasin D (Synonyms: 細胞松弛素D; Zygosporin A; NSC 209835)
生物活性
Cytochalasin D (Zygosporin A) is a potent actin polymerization inhibitor, could be derived from fungus. Cytochalasin D has cell-permeable activity. Cytochalasin D inhibits the G-actin–cofilin interaction by binding to G-actin. Cytochalasin D also inhibits the binding of cofilin to F-actin and decreases the rate of both actin polymerization and depolymerization in living cells. Cytochalasin D can reduce exosome release, in turn reducing the amount of survivin present in the tumour environment. Cytochalasin D induces phosphorylation and cytoplasmic retention of Yap
體外研究(In Vitro)
Cytochalasin D (3 and 10 μM; 30 min) causes retraction and arborization of COS-7 cells and conversion of F-actin from long fibers to punctate structures[1].
Cytochalasin D (0.3, 1, 3 and 10 μM; 30 min) concentration-dependently decreases the rate of actin depolymerization in COS-7 cells[1].
Cytochalasin D (1 μM; NIH3T3 cells) disrupts stress fibers, induces phosphorylation of Yap, while cells maintains original area and lost nuclear Yap localization
| Cell Line: | COS-7 cells[1] |
| Concentration: | 3 and 10 μM |
| Incubation Time: | 30 min |
| Result: | Caused retraction and arborization of COS-7 cells and conversion of F-actin from long fibers to punctate structures. |
分子量：507.62
Formula：C30H37NO6
CAS 號：22144-77-0
中文名稱：細胞松弛素D
運輸條件：Room temperature in continental US; may vary elsewhere.
儲存方式：
| Powder | -20°C | 3 years |
|---|---|---|
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
溶解性數據
DMSO : 25 mg/mL (49.25 mM; ultrasonic and warming and heat to 60°C)
| 濃度溶劑體積質量 | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.9700 mL | 9.8499 mL | 19.6998 mL |
| 5 mM | 0.3940 mL | 1.9700 mL | 3.9400 mL |
| 10 mM | 0.1970 mL | 0.9850 mL | 1.9700 mL |
請根據產品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 儲存時，請在 6 個月內使用，-20°C 儲存時，請在 1 個月內使用。
參考文獻
[1]. Shoji K, et, al. Cytochalasin D acts as an inhibitor of the actin-cofilin interaction. Biochem Biophys Res Commun. 2012 Jul 20;424(1):52-7.
[2]. Flanagan MD, et, al. Cytochalasins block actin filament elongation by binding to high affinity sites associated with F-actin. J Biol Chem. 1980 Feb 10;255(3):835-8.
[3]. Catalano M, et, al. Inhibiting extracellular vesicles formation and release: a review of EV inhibitors. J Extracell Vesicles. 2019 Dec 19;9(1):1703244.
[4]. Wada K, et, al. Hippo pathway regulation by cell morphology and stress fibers. Development. 2011 Sep;138(18):3907-14.
注：產品僅用于科研
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