Bovine Serum Albumin (Synonyms: 白蛋白; Albumin bovine serum; BSA)
Bovine Serum Albumin (BSA) 是由三個同源全 α 結(jié)構(gòu)域組成的 583 個氨基酸的蛋白質(zhì)。BSA 是一種球狀蛋白,可用于多種生物化學(xué)實驗。
生物活性
Bovine Serum Albumin (BSA) is a 583-residue protein consisting of three homologous all-α domains, organized in a heart-shaped structure. BSA is a globular protein that is used in numerous biochemical applications.
體外研究(In Vitro)
Bovine serum albumin (BSA) is a 583-residue protein consisting of three homologous all-α domains, organized in a heart-shaped structure. Bovine serum albumin constitutes ca. 60% of all plasma protein and binds and transports a large number of physiological and non-physiological ligands. Bovine serum albumin contains 17 disulfide bonds and one unpaired cysteine (Cys34), which facilitates dimerization and also influences higher-order association, since the rate of aggregation is slowed down if Cys34 is covalently bound to another compound. MTT assay for fibril cytotoxicity shows Bovine Serum Albumin is beneficial for cell growth irrespective of its aggregated state. Lack of cytotoxicity is confirmed by membrane permeabilization assays. In the subsequent 40 h, an increase in the viability of the treated cells of 300-400% is observed, indicating that the cells incubated with Bovine Serum Albumin achieve a higher viability than the untreated cells[1].
性狀:Solid
CAS 號:9048-46-8
中文名稱:牛血清白蛋白;牛白蛋白;卵蛋白粉;血清蛋白;血清白蛋白;牛血蛋白質(zhì);牛血清蛋白;牛血清蛋白;牛血清清蛋白;白蛋白
運輸條件:Room temperature in continental US; may vary elsewhere.
儲存方式
4°C, protect from light
*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶解性數(shù)據(jù)
In Vitro:
H2O : 50 mg/mL (Need ultrasonic)
以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液,再依次添加助溶劑:
——為保證實驗結(jié)果的可靠性,澄清的儲備液可以根據(jù)儲存條件,適當(dāng)保存;體內(nèi)實驗的工作液,建議您現(xiàn)用現(xiàn)配,當(dāng)天使用; 以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比;如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象,可以通過加熱和/或超聲的方式助溶
- 1.
請依序添加每種溶劑: PBS
Solubility: 100 mg/mL (Infinity mM); Clear solution; Need ultrasonic
注:產(chǎn)品僅用于科研