運(yùn)用高通量熒光定量PCR芯片方法,在一張96孔或384孔板上同時(shí)對(duì)某個(gè)信號(hào)通路或疾病相關(guān)基因的多個(gè)基因的表達(dá)量變化進(jìn)行檢測(cè),芯片上的基因包括了與研究對(duì)象有確定關(guān)系的基因或者待考證的基因;目前啟因生物針對(duì)不同的信號(hào)通路和疾病相關(guān)基因設(shè)計(jì)了上百種多款功能分類芯片,涵蓋生命科學(xué)研究的各個(gè)領(lǐng)域。
Real-time PCR arrays are tools that allow convenient testing of samples in many assays concurrently, parallel testing of many samples or testing of multiple samples and targets simultaneously.
——Saunders N A(2013)
品牌 | 貨號(hào) | 名稱 | 基因列表 | 價(jià)格 |
啟因生物(wcgene.com) | HUMAN-WC093 | Other Genes Associated with MSC qpcr array | ANXA5, BDNF, BGLAP (Osteocalcin), BMP7, COL1A1, CSF2 (GM-CSF), CSF3 (GCSF), CTNNB1, EGF, FUT1, GTF3A, HGF (Scatter Factor),ICAM1, IFNG, IGF1, IL10, IL1B, IL6, ITGB1, KITLG, MMP2, NES, NUDT6, PIGS, PTPRC, SLC17A5, TGFB3, TNF, VEGFA, VIM, VWF. | 300元/樣 |
啟因生物(wcgene.com) | MOUSE-WC092 | Other Genes Associated with MSC qpcr array | Anxa5, Bdnf, Bglap, Bmp7, Col1a1, Csf2, Csf3, Ctnnb1, Egf, Fut1, Gtf3a, Hgf, Icam1, Ifng, Igf1, Il10,Il1b, Il6, Itgb1, Kitl, Mitf, Mmp2, Nes, Nudt6, Pigs, Ptprc, Slc17a5, Tgfb3, Tnf, Vegfa, Vim, Vwf. | 300元/樣 |
啟因生物(wcgene.com) | RAT-WC094 | Other Genes Associated with MSC qpcr array | Anxa5, Bdnf, Bglap (Osteocalcin), Bmp7, Col1a1, Csf2 (GM-CSF), Csf3 (GCSF), Ctnnb1, Egf, Fut1, Hgf (Scatter Factor), Icam1,Ifng, Igf1, Il10, Il1b, Il6, Itgb1, Kitlg, Mitf, Mmp2 (Gelatinase A), Nes, Nudt6, Pigs, Ptprc, Slc17a5, Tgfb3, Tnf, Vegfa, Vim, Vwf. | 300元/樣 |
檢測(cè)流程: 您只需提供樣品,其他工作交給我們。
1. 樣品RNA提取;
2. RNA質(zhì)量檢測(cè),檢測(cè)RNA純度及完整度,提供RNA質(zhì)量報(bào)告;
3. cDNA合成,使用逆轉(zhuǎn)錄酶,將樣品RNA逆轉(zhuǎn)錄為cDNA;
4. 實(shí)時(shí)定量PCR反應(yīng);
5. 數(shù)據(jù)分析,計(jì)算PCR芯片中的各個(gè)Ct值,采用ΔΔCt方法比較基因的表達(dá)變化,并利用公司自主開發(fā)的數(shù)據(jù)分析軟件進(jìn)行比較。