補(bǔ)體片斷5b（C5b）ELISA檢測(cè)試劑盒齊一生物的ELISA試劑盒是您的*選擇:每個(gè)試劑盒都經(jīng)過嚴(yán)格的質(zhì)檢控制，包括：板內(nèi)、板間實(shí)驗(yàn)精度，靈敏度，回收率，穩(wěn)定性等指標(biāo)的測(cè)定。我們提供的96孔板已經(jīng)預(yù)包被好捕獲抗體，三步法檢測(cè)體系簡(jiǎn)單而直接，可根據(jù)450 nm波長(zhǎng)的讀值迅速計(jì)算定量結(jié)果。試劑盒中提供的檢測(cè)抗體能夠識(shí)別全長(zhǎng)靶蛋白的抗原決定簇以及它們的三維結(jié)構(gòu)，使您的檢測(cè)結(jié)果更穩(wěn)定可靠
補(bǔ)體片斷5b（C5b）ELISA檢測(cè)試劑盒一、高效、靈敏、特異的抗體；
二、穩(wěn)定的重復(fù)性和可靠性；
三、吸附性能好,空白值低,孔底透明度高的固相載體；
四、適用血清、血漿、組織勻漿液、細(xì)胞培養(yǎng)上清液、尿液等等多種標(biāo)本類型；
五、節(jié)省實(shí)驗(yàn)經(jīng)費(fèi).
elisa試劑盒回收率是反應(yīng)待測(cè)物在樣品分析過程中的損失的程度,損失越少,回收率越高,如果作標(biāo)液1PPM,就是1毫克/升,而作出標(biāo)準(zhǔn)數(shù)據(jù)為0.99毫克/升,就是說(shuō)你的回收率是99%,這個(gè)與真實(shí)成分有密切的關(guān)系,說(shuō)明方法的準(zhǔn)確度.比如水中總無(wú)機(jī)氯含量測(cè)定,樣品水中含有無(wú)機(jī)氯20mg/L,取100mL被測(cè)水樣品,加入0.1mL濃度為10mg/mL的含無(wú)機(jī)氯標(biāo)準(zhǔn)樣品,測(cè)定時(shí)忽略體積變化,如果測(cè)定出樣品中無(wú)機(jī)氯為2.98mg/L,則認(rèn)為回收率為99%.
制備方法
包括以下步驟：
1)抗原表位的計(jì)算機(jī)篩選；
2)抗原的制備；
3)血清的采集；
4)間接ELISA方法的建立；
5)間接ELISA方法的敏感性和特異性驗(yàn)證；
6)試劑盒的穩(wěn)定性驗(yàn)證；
7)對(duì)屠宰場(chǎng)進(jìn)行臨床檢測(cè).該方法簡(jiǎn)單、快速、靈敏度高、特異性強(qiáng)、穩(wěn)定性合格、重復(fù)性好.應(yīng)用本發(fā)明制得的試劑盒能有效檢出感染豬的戊型肝炎病毒,適用于大批量發(fā)病樣本的檢測(cè),可用于豬戊肝的快速診斷,并預(yù)防、控制豬戊肝病毒的流行和阻斷戊肝病毒由豬向人傳播.
組成結(jié)構(gòu)
1、血清：操作過程中避免任何細(xì)胞刺激.使用不含熱原和內(nèi)毒素的試管.收集血液后,1000×g離心10分鐘將血紅細(xì)胞迅速小心地分離.
2、血漿：EDTA、檸檬酸鹽、肝素血漿可用于檢測(cè).1000×g離心30分鐘去除顆粒.
3、細(xì)胞上清液：1000×g離心10分鐘去除顆粒和聚合物.
4、組織勻漿：將組織加入適量生理鹽水搗碎.1000×g離心10分鐘,取上清液.
5、保存：如果樣品不立即使用,應(yīng)將其分成小部分-70℃保存,避免反復(fù)冷凍.盡可能的不要使用溶血或高血脂血.如果血清中大量顆粒,檢測(cè)前先離心或過濾.不要在37℃或更高的溫度加熱解凍.應(yīng)在室溫下解凍并確保樣品均勻地充分解凍.
此IBL試劑盒能用于小鼠血清,EDTA血漿,細(xì)胞上清中產(chǎn)品定量檢測(cè)試劑盒成分
1預(yù)包被板:抗小鼠白介素-6兔子IgG,親合純化96T
2酶標(biāo)記抗體:(30倍濃縮)HRP標(biāo)記抗小鼠白介素-6兔子IgG,親合純化0.4mLx1
3標(biāo)準(zhǔn)品:重組小鼠白介素-60.5mLx2
4EIA緩沖液:含1%BSA,0.05%吐溫20BPS30mLx1
5標(biāo)記抗體稀釋液:含1%BSA,0.05%吐溫20BPS12mLx1
6顯色劑:TMB底物液15mLx1
7終止液:1N硫酸12mLx1
8濃縮洗滌液:(40倍濃縮)含1%BSA,0.05%吐溫20BPS50mLx1操作說(shuō)明1實(shí)驗(yàn)所需器材(但試劑盒沒有提供)酶標(biāo)儀(450nm)微移液管及其吸嘴量筒及燒杯去離子水冰箱(4°C)坐標(biāo)紙(log/log)吸水紙?jiān)嚬?用于標(biāo)準(zhǔn)品稀釋)溫育箱(37°C±1°C)洗瓶(用于洗板)一次性試劑管(用于濃縮酶標(biāo)記抗體和顯色劑)
MS IGG1 KPA ITCL NHP FITC 50TST MOPC-21 551954
MS CXCR5 PE MAB 0.1MG 2G8 551959
MS CXCR5 BIOTIN MAB 0.1MG 2G8 551960
MS CXCR5 PURE MAB 0.1MG 2G8 551961
NF-M P-SPEC PURE MAB W/CONT 50UG RNF406 551962
HU TLR4 PURE MAB 0.1MG HTA125 551964
MS CD184 PE MAB 0.1MG 2B11/CXCR4 551966
MS CD184 FITC MAB 0.1MG 2B11/CXCR4 551967
MS CD184 BIOTIN MAB 0.1MG 2B11/CXCR4 551968
MS CD212 BIOTIN MAB 0.1MG 114 551973
MS CD212 PE MAB 0.1MG 114 551974
HU TLR4 BIOTIN MAB 100TST HTA125 551975
RAT IGG1 KPA ITCL NHP PE MAB 50TST R3-34 551979
HU CD4 NHP APC MAB 50TST L200 551980
RAT CD147 PURE MAB 0.1MG OX-47 552012
MS LY-49A BIOTIN MAB 0.1MG JR9-318 552014
MS CD278 PURE MAB 0.5MG 7E.17G9 552015
LUMINOMETER INJECTION CLEANING SOLUTION 552017
HU CD200 PURE MAB 0.1MG MRC OX-104 552023
HU CD172B PURE MAB 0.1MG B4B6 552024
HU CD138 PE MAB 100TST MI15 552026
HU CXCR5 PURE MAB 0.1MG RF8B2 552032
HU TLR1 PURE MAB 0.1MG GD2 552033
FUT-175 (FUTHAN) LYO 5.0MG 552035
MS CD4 APC-CY7 MAB 0.1MG GK1.5 552051
HU CD14 NALE MAB 0.5MG 3-C39 552052
CDK8 PURE MAB 150UG F9-1075 552053
CDK8 PURE MAB 50UG F9-1075 552056
MS LY-6G LY-6C PERCP-CY5.5 0.1MG RB6-8C5 552093
MS CD45R APC-CY7 MAB 0.1MG RA3-6B2 552094
HU CXCR5 BIOTIN MAB 100TST RF8B2 552118
HU CD124 ALP BIOTIN MAB 100TST HIL4R-M57 552120
MS SIGLEC-F PURE MAB 0.1MG E50-2440 552125
MS SIGLEC-F PE MAB 0.1MG E50-2440 552126
HU CD3 NHP PE MAB 50TST SP34-2 552127
MS CD180 PURE MAB 0.5MG RP/14 552128
MS CD180 BIOTIN MAB 0.5MG RP/14 552129
RAT MYELOID LINEAGE PURE MAB 0.1MG OX-82 552130
MS CTLA-4 IGG NALE FUSION PTEIN 0.5MG 552133
HU IFN-GMA ELISPOT KIT 2PLT 552138
MS V DTA 4 TCR FITC MAB 0.1MG GL2 552143
HAM IGG3 KPA ITCL PE MAB 0.1MG E36-239 552144
MS CD278 BIOTIN MAB 0.5MG 7E.17G9 552145
MS CD278 PE MAB 0.2MG 7E.17G9 552146
QIAsoft 4.1.4.8 Servicepack 9016114
Board, YZ6L, master, BR8 AT3/B10 9016048
Software QIAsoft MDx Service Edition 9015913
Fittings (10), M6/2.8 mm, white 9015686
Board, power branching 9015528
Board, operation panel 9015527
Vacuum Pump (100 V), PyroQ24VW 9021234
Skin Panel, Waste Drawer, DCU 9021114
Skin Panel, Lower Center Rear, DCU 9021100
Vial Robot Assist Assembly, DCU 9020996
Kit, calibration, mech. variation, RG3 9020937
Pulley, x idler belt tensioner, C34 9020708
Spindle nut - XY, PyroQ24 9020691
Display, keypad, GSC 9020487
Kit, fuse AS, Qsym 9020390
PM kit, C12 & QIAgility, CG1 9020316
Packaging, foam base, C1x 9020265
Z-pulley, idler ass., 8 ch. gearbox, Cx8 9020234
Belt, Y-axis assembly, Cx8 9020228
Fitting,Peek,10-32,1/16tubing (5) 9239525
Vacuum Pump (110-120 V), PyroQ24VW 9020164
Mikrowin data reduction Software 9232820
Cal. Set for Reader 3 000 12 00 9232807
Manual, user, QS 4.1 (WW) 9236528
#Agreement, Plato 3, PM, 1 yr 9236486
Travel Charge, zone A 9236446
1.0 hr 9236439
Holder, tip rack 2 9011884
Power Supply, 400W, BR8, RD v1.0 9236982
X/Y table, PyroQ96ID 9019142
Camera MX5, PyroQ96ID 9019128
Heating block, PyroQ96ID 9019127
Pin, piercing, EZ1 9019027
Worktable, side wall, right, EZ1xl 9019026
Master Mix Block, 5x5ml Tubes 9018959
Reagent Block, 16x0.2ml/1.5ml PCR/FlatBa 9018952
Adapter, COBAS TaqMan 48 Plates 9018941
Precision Pipetting Calibration Kit 9015617
EZ1 RNA Card 9015590
Cover, back ,type C 9015533
Door Lock Upgrade Kit, BR EZ1 9015520
Tube Holder, STS, 5ml, 16 pos (6) 9014187
RoboVac, Base, BR8 RD, v4.2 9014154
Belt, Y-drive, Mounting Tool, BR8000 9013993
Controller, Z Axis, VRA, DCU 9021177
Output Rack, 96 pos, DCU 9021162
Door Assy, Upper Left, DCU 9021058
Controller, Theta Rotary, DCU 9021013
Belt, X Drive, DCU 9021001
PCB, Pipettor, DCU 9020998
Pip. head, non LLS, 1ch. (EXCHANGE), CC2 9020770
Lid, polycarbonate with UV, C34 9020717
Shipment Box, PyroQ24 9020693
Waste chute, bent, SP, Cabinet Qsym 9020644
Door, waste, AS, Cabinet Qsym 9020630
Power supply, AS, Qsym 9020397
Cable - USB, RGQII 9020346
Pipetting head, 8 ch. (EXCHANGE), CX1 9020337
PCB - Mainboard, RGQII 9020330
Cable - LED, RGQII 9020329
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